The Granier-Mouillac Lab aims at characterizing the molecular and structural mechanisms of GPCRs and PAQRs function. Both of these receptor families present a 7TM which acts as a receptor and a transducer of extracellular signals within the cell to regulate a wide variety of physiological function.
As such, they are the subject of intense studies both in academic labs and in industry with important drug discovery programs. Despite recents advance in the field, there is still an important lack of knowledge of 7TM signalling mechanisms and pharmacology. This is mainly due to the challenges associated with the characterisation of membrane protein structure and function.
Integrated Structural Biology Approachs
Structural analyses of receptors and signaling complexes using biochemistry, fluorescence, NMR spectroscopy, X-ray crystallography, cryo-Electron Microscopy ans Native Mass Spectrometry.
BRET / FRET assays
Xray Crystallography
Cryo-EM
Native MS
solution NMR
Molecular Dynamics
Protein engineering
Chemical engineering
Bacterial expression
